Axis BTA 2100D Návod k obsluze stáhnout pdf (Strana 89)

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is outside of these limits, check if the rf voltage amplitude is adjusted properly

over the entire mass range).

Straight Line Mass Calibration

To initiate a straight line mass calibration, click Straight Line Calibration and Start

Auto Tune. A straight line calibration assumes a linear relationship between DAC

steps and mass. It is used for certain hardware adjustments. When you initiate

the program, a straight line calibration curve is calculated with the zero DAC step

set equal to 0 m/z and 4095th DAC step set equal to 650 m/z.

Trap Function Calibration

When the mass calibration has completed, the trap function calibration can be

performed, if an MS/MS or SIS key is installed on the SAP/Wave Board. This

calibration determines parameters which are required for the operation of ion

preparation methods such as MS/MS and SIS. It takes several minutes to

complete and the Trap Function calibration should be done each time a mass

calibration is done. If you are not using any ion preparation methods, the trap

function calibration does not have to be done. However, before you activate an

ion preparation method, you must then repeat the mass calibration with a trap

function calibration.

At the completion of Trap Function Calibration, the Event Message Window

displays the secular frequency values for the two trap function calibration points.

Nominal values are 257 and 255, respectively. These values can vary up to 4

KHz between instruments.

Set Points

The electron multiplier voltage and the SIS amplitude adjust factor can be set by

either changing the value in the edit box or by clicking on the up/down arrows.

The values are not set until the apply button is clicked and will be reset to their

previous values if the setpoints tab is left before clicking the apply button. If one

of the edit controls has the focus, the enter key can be pressed instead of the

apply button.

Electron Multiplier Voltage. Final Gain Setting determine from the Auto Tune

check of EM.

1 2 ... 84 85 86 87 88 89 90 91 92 93 94 ... 596 597

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